Functional Genomics Lab

Pricing

We offer two options for Illumina HiSeq 1000 and NextSeq 500 sequencing:

• Illumina library prep + sequencing
• Only sequencing

Illumina library prep

• RNASeq (eukariotic): €250 per library (at least 4 samples, less than 4 price on request)
• gDNA (eukariotic, bacterial): €250 per library (at least 4 samples, less than 4 price on request)
• Mate-Pairs (from 3Kb to 10 Kb): €1.150 per library (1 size), €1.200 per library (2 sizes), €1.250 per library (3 sizes).

HiSeq 1000 sequencing service using v.3 clusters and sequencing kits

• Single read sequencing, 50nt reads: (only on request for a complete flow cell) €1.500 per lane
• Single read sequencing, 100nt reads: (only on request for a complete flow cell) €2.000 per lane
• Paired-end sequencing, 100nt reads: (only on request for a complete flow cell) €3.500 per lane

Average number of fragments sequenced = 180.000.000

NextSeq 500 Sequencing service using v.2 sequencing kits

• HighOutput – 300 cycles: (only on request for a complete flow cell) €8.000 per flow cell
• HighOutput – 150 cycles: (only on request for a complete flow cell) €5.000 per flow cell
• HighOutput – 75 cycles: (only on request for a complete flow cell) €2.800 per flow cell
• MidOutput – 300 cycles: (only on request for a complete flow cell) €3.500 per flow cell
• MidOutput – 300 cycles: (only on request for a complete flow cell) €2.200 per flow cell

Average number of fragments sequenced = HighOutput 400.000.000 / MidOutput 130.000.000

Exome sequencing

Kit	Max. num. of samples per capture	Price per capture
Illumina Nextera Exome Enrichment Kit (without UTRs)	From 1 to 3	€1.100
Illumina Nextera Exome Enrichment Kit (without UTRs)	From 1 to 12	€2.800
Illumina Nextera Exome Expanded Enrichment Kit (with UTRs)	From 1 to 12	€3.300
Nimblegen SeqCap EZ Human Exome Library v3.0 (without UTRs)	From 1 to 12	€2.300
Nimblegen SeqCap EZ Exome (with UTRs)	From 1 to 12	€2.300
Agilent SureSelect XT Human All Exon V5 (with UTRs)	1	€1.000

Exome sequencing coverage per sample will be evaluated on request.

Bioinformatics

RNA-Seq data analyses

• Digital Expression analysis (data QC, mapping on reference genome, evaluation of expression level, differential expression analysis)
• Digital Expression analysis + identification of novel genes and isoforms
• Transcriptome assembly, evaluation of expression levels and differential expression analysis

DNA-Seq data analyses

• Annotation
• De novo assembly

Costs will be evaluated on request.

Exome data analysis (per exome)

Type of Analysis	Price
Alignment and variant calling	€300
Alignment, variant calling, annotation with prediction software, conservation and frequency in the population	€500

For further information please contact us to discuss how we can assist you in achieving your project goals and to receive a quote for your project.

Facility users other than University of Verona are subject to a 22% VAT. For collaborations a 12% discount is applied. For collaborations with groups of the University of Verona a further 10% discount is applied.