The Functional Genomics Center (FGC) is located in the Department of Biotecnology of University of Verona. Established in 2007 by Massimo Delledonne and Mario Pezzotti , the FGC operates in the area of transcriptomics analysis in plants, animals, bacteria. With the advent of Next Generation Sequencing Technologies, the FGC expanded its activities to genome assembly, genome resequencing and RNA-Seq analysis. This center is now dedicated to the development and use of the new tools that the genomic technologies are now offering to microbial, plant, animal and human biologists.
Important!: As of December of 2012 the Functional Genomics Lab is no longer offering services based on microarray platforms. If you are interested into microarray analysis applied to Grape transcriptomics please contact Prof. Mario Pezzotti.
We offer two options for Illumina HiSeq 1000 sequencing:
| Description | Price (VAT excluded) |
|---|---|
| RNA sample QC with Bioanalyzer platform (up to 12 samples) | €50 |
| Standard TruSeq mRNA library | €250 |
| Directional TruSeq mRNA library (Poly(A+) enriched) | €270 |
| Directional TruSeq RNA library (Ribozero Gold) | €410 |
| Directional TruSeq RNA library (Ribozero Plant) | €410 |
| Small RNA library | €270 |
| Standard TruSeq DNA library | €250 |
| ChIPSeq library | €205 |
| Mate-pair library (2-8Kb) | €970 |
Facility users other than University of Verona are subject to a 22% VAT. For collaborations a 12% discount is applied. For collaborations with groups of the University of Verona a further 10% discount is applied.
| Description | Price (VAT excluded) |
|---|---|
| 1 lane, 100nt paired-end sequencing (100nt X 2) | €3100 |
| 1 lane, 50nt paired-end sequencing (50nt X 2)* | €2250 |
| 1 lane, 100nt single-read sequencing* | €1800 |
| 1 lane, 50nt single-read sequencing* | €1400 |
* Formats available on request only on a per complete flowcell (7 lanes + 1 control lane) basis.
Usual number of fragments sequenced = 160,000,000
| Kit | Sample multiples | Expected theoretical average read depth | Observed average read depth of target regions | CDS | UTR | Target size | Service price |
|---|---|---|---|---|---|---|---|
| Nextera rapid capture expanded exome | 6 | 190-200X | 80X | YES | YES | 62Mb | 1400 |
| Nextera rapid capture expanded exome | 6 | 95-100X | 40X | YES | YES | 62Mb | 950 |
| Nextera rapid capture exome | 5 | 190-200X | 80X | YES | NO | 37Mb | 900 |
| Nextera rapid capture exome | 10 | 95-100X | 40X | YES | NO | 37Mb | 600 |
Facility users other than University of Verona are subject to a 22% VAT. For collaborations a 12% discount is applied. For collaborations with groups of the University of Verona a further 10% discount is applied.
| Description | Price (VAT excluded) |
|---|---|
| Reads alignment, variant calling and annotation of variants with Annovar (1 exome) | €300 |
| Reads alignment, variant calling, analysis with Knome or GoldenHelyx SVS software. | €500 |
| Description | Price (6 samples per lane; VAT excluded) | Price (3 samples per lane; VAT excluded) |
|---|---|---|
| Digital Expression analysis (data QC, mapping on reference genome, evaluation of expression level, differential expression analysis) | €1150 (per lane) | €750 (per lane) |
| Digital Expression analysis + identification of novel genes and isoforms | €1500 (per lane) | €950 (per lane) |
| Transcriptome assembly, evaluation of expression levels and differential expression analysis | €2300 (per lane) | €1500 (per lane) |
Facility users other than University of Verona are subject to a 22% VAT. For collaborations a 12% discount is applied. For collaborations with groups of the University of Verona a further 10% discount is applied.
Prices are subject to change at any time. For further information please contact us to discuss how we can assist you in achieving your project goals and to receive a quote for your project.
Department of Biotechnologies, University of Verona
Strada le Grazie, 15 - Cà Vignal, 1 - 37134 (VR) - Italy
Phone: +39 045 802 7058
Fax: +39 045 802 7929